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h pylori strain 60190  (ATCC)


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    Structured Review

    ATCC h pylori strain 60190
    H Pylori Strain 60190, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h+pylori/Helicobacter+Pylori%3B+Strain%3A+60190/pmc12922380-86-13-20
    Average 95 stars, based on 461 article reviews
    h pylori strain 60190 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Injection:

    Article Title: Activated sympathetic nerve post stroke downregulates Toll-like receptor 5 and disrupts the gut mucosal barrier
    Article Snippet: .. For MMT of H.pylori , 100 μL of 1∗10 9 cfu/mL H.pylori (ATCC 43504) was administered to mice by injection into the proximal colon. ..

    Article Title: Activated sympathetic nerve post stroke downregulates Toll-like receptor 5 and disrupts the gut mucosal barrier.
    Article Snippet: .. For MMT of H.pylori, 100 mL of 1*109 cfu/mL H.pylori (ATCC 43504) was administered to mice by injection into the proximal colon. ..

    Capsules:

    Article Title: Twice-a-day PPI, tetracycline, metronidazole quadruple therapy with Pylera® or Lactobacillus reuteri for treatment naïve or for retreatment of Helicobacter pylori. Two randomized pilot studies.
    Article Snippet: Correspondence Maria Pina Dore, Clinica Medica, Universita` di Sassari, Viale San Pietro, 8, Sassari 07100, Italy.. Email: mpdore@uniss.it Abstract Background: Bismuth is no longer available in Europe except as part of combination therapy.. Lactobacillus reuteri has also been used as an adjuvant for Helicobacter pylori therapy.

    Incubation:

    Article Title: Toll-Like Receptor 1 Locus Re-examined in a Genome-Wide Association Study Update on Anti– Helicobacter pylori IgG Titers
    Article Snippet: .. After 24 hours of incubation, wells were washed and PBMCs stimulated with 1 million colony-forming units of heat-killed H pylori (strain ATCC-43504 [cagA + , vacA (s1/m1) , iceA + , babA2 + ]; Manassas, VA) grown on Trypticase Soy Agar (Oxoid, Hampshire, UK) supplemented with 5% defibrinated sheep blood (VWR, Radnor, PA) and DENT selective medium (Oxoid). .. Other stimuli used were TLR1 inhibitor CU-CPT-22 (5 μ mol/L; Tocris Bioscience, Bristol, UK) and TLR1 agonist Pam3Cys4 (300 ng/mL; InvivoGen, San Diego, CA).

    Article Title: Toll-Like Receptor 1 Locus Re-examined in a Genome-Wide Association Study Update on Anti-Helicobacter pylori IgG Titers.
    Article Snippet: .. After 24 hours of incubation, wells were washed and PBMCs stimulated with 1 million colony-forming units of heat-killed H pylori (strain ATCC-43504 [cagAþ, vacA(s1/m1), iceAþ, babA2þ]; Manassas, VA) grown on Trypticase Soy Agar (Oxoid, Hampshire, UK) supplemented with 5% defibrinated sheep blood (VWR, Radnor, PA) and DENT selective medium (Oxoid). .. Other stimuli used were TLR1 inhibitor CU-CPT-22 (5 mmol/L; Tocris Bioscience, Bristol, UK) and TLR1 agonist Pam3Cys4 (300 ng/ mL; InvivoGen, San Diego, CA).47 Supernates were harvested after 8 hours of stimulation for ELISA experiments unless otherwise specified to measure tumor necrosis factor (TNF) a, interleukin-8 (IL8), and IL10 (eBioscience, San Diego, CA) as described previously.48 All samples were tested in duplicate.

    Article Title: IL-6 facilitates cross-talk between epithelial cells and tumor- associated macrophages in Helicobacter pylori -linked gastric carcinogenesis
    Article Snippet: .. Following a 24-hour incubation period, medium was refreshed and cells were stimulated with recombinant human IL6 (50 ng/mL; InvivoGen, San Diego, CA) or 10 6 colony-forming units (CFU) of heat-killed H pylori (strain ATCC-43504 [cagA+, vacA(s1/m1), iceA+, and babA2+]; Manassas,VA). .. IL-6 production in supernatants was determined at 6, 24, 48, 72, 96, and 120 h of stimulation by enzyme linked immunosorbent assay (ELISA) (eBioscience, San Diego, CA), as per manufacturer's instructions.

    Transferring:

    Article Title: Synthesis and biological evaluation against H. pylori of chitosan menthone Schiff base hybrid with different types of inorganic nanoparticles.
    Article Snippet: The production of novel natural medicines for the treatment of Helicobacter pylori (H. pylori) has lately attracted a lot of interest.. Some bacterial infections have traditionally been alleviated by terpenes.. The present work intended to examine the impact of several chitosan menthone Schiff base nanocomposites on the treatment of H. pylori infection as well as on its anti-inflammatory capacity.

    Sterility:

    Article Title: Synthesis and biological evaluation against H. pylori of chitosan menthone Schiff base hybrid with different types of inorganic nanoparticles.
    Article Snippet: The production of novel natural medicines for the treatment of Helicobacter pylori (H. pylori) has lately attracted a lot of interest.. Some bacterial infections have traditionally been alleviated by terpenes.. The present work intended to examine the impact of several chitosan menthone Schiff base nanocomposites on the treatment of H. pylori infection as well as on its anti-inflammatory capacity.

    Saline:

    Article Title: Synthesis and biological evaluation against H. pylori of chitosan menthone Schiff base hybrid with different types of inorganic nanoparticles.
    Article Snippet: The production of novel natural medicines for the treatment of Helicobacter pylori (H. pylori) has lately attracted a lot of interest.. Some bacterial infections have traditionally been alleviated by terpenes.. The present work intended to examine the impact of several chitosan menthone Schiff base nanocomposites on the treatment of H. pylori infection as well as on its anti-inflammatory capacity.

    Suspension:

    Article Title: Synthesis and biological evaluation against H. pylori of chitosan menthone Schiff base hybrid with different types of inorganic nanoparticles.
    Article Snippet: The production of novel natural medicines for the treatment of Helicobacter pylori (H. pylori) has lately attracted a lot of interest.. Some bacterial infections have traditionally been alleviated by terpenes.. The present work intended to examine the impact of several chitosan menthone Schiff base nanocomposites on the treatment of H. pylori infection as well as on its anti-inflammatory capacity.

    Recombinant:

    Article Title: IL-6 facilitates cross-talk between epithelial cells and tumor- associated macrophages in Helicobacter pylori -linked gastric carcinogenesis
    Article Snippet: .. The cells were then stimulated for 24 h with recombinant human IL6 (50 ng/mL; InvivoGen, San Diego, CA) or 10 6 CFU of heat-killed H pylori (strain ATCC-43504, Manassas,VA). ..

    Article Title: IL-6 facilitates cross-talk between epithelial cells and tumor- associated macrophages in Helicobacter pylori -linked gastric carcinogenesis
    Article Snippet: .. Following a 24-hour incubation period, medium was refreshed and cells were stimulated with recombinant human IL6 (50 ng/mL; InvivoGen, San Diego, CA) or 10 6 colony-forming units (CFU) of heat-killed H pylori (strain ATCC-43504 [cagA+, vacA(s1/m1), iceA+, and babA2+]; Manassas,VA). .. IL-6 production in supernatants was determined at 6, 24, 48, 72, 96, and 120 h of stimulation by enzyme linked immunosorbent assay (ELISA) (eBioscience, San Diego, CA), as per manufacturer's instructions.



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    SCFAs induce CAPZA1 expression in AGS cells and promote CagA accumulation and CD44v expression under H pylori infection. (A) Western blotting analysis of CAPZA1 expression in AGS cells treated with propionate (Pro) or butyrate (But), with signal quantification using ImageJ software. (B) HDAC activity in AGS cells treated with Pro or But. Statistical analysis was performed using one-way ANOVA followed by Tukey’s test. ∗ P < .05, ∗∗ P < .01. (C) ChIP assay showing histone acetylation in the CAPZA1 promoter region of AGS cells treated with Pro or But. Chromatin was immunoprecipitated with anti-histone H3 (acetyl K9) or control IgG, and enrichment of CAPZA1 promoter regions was assessed using real-time PCR. (D) Immunofluorescence analysis of autolysosome formation in But-treated AGS cells infected with H pylori ATCC 700392. Lysosomes and nuclei were stained with LysoTracker and 4ʹ,6-diamidino-2-phenylindole, respectively. Scale bar = 50 μm. (E) Western blotting analysis of CagA accumulation in But-treated AGS cells infected with H pylori ATCC 700392. (F) Western blotting analysis of CD44v expression in Pro- or But-treated AGS cells infected with H pylori G27 or G27 Δ cag PAI strains.
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    SCFAs induce CAPZA1 expression in AGS cells and promote CagA accumulation and CD44v expression under H pylori infection. (A) Western blotting analysis of CAPZA1 expression in AGS cells treated with propionate (Pro) or butyrate (But), with signal quantification using ImageJ software. (B) HDAC activity in AGS cells treated with Pro or But. Statistical analysis was performed using one-way ANOVA followed by Tukey’s test. ∗ P < .05, ∗∗ P < .01. (C) ChIP assay showing histone acetylation in the CAPZA1 promoter region of AGS cells treated with Pro or But. Chromatin was immunoprecipitated with anti-histone H3 (acetyl K9) or control IgG, and enrichment of CAPZA1 promoter regions was assessed using real-time PCR. (D) Immunofluorescence analysis of autolysosome formation in But-treated AGS cells infected with H pylori ATCC 700392. Lysosomes and nuclei were stained with LysoTracker and 4ʹ,6-diamidino-2-phenylindole, respectively. Scale bar = 50 μm. (E) Western blotting analysis of CagA accumulation in But-treated AGS cells infected with H pylori ATCC 700392. (F) Western blotting analysis of CD44v expression in Pro- or But-treated AGS cells infected with H pylori G27 or G27 Δ cag PAI strains.
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    Image Search Results


    SCFAs induce CAPZA1 expression in AGS cells and promote CagA accumulation and CD44v expression under H pylori infection. (A) Western blotting analysis of CAPZA1 expression in AGS cells treated with propionate (Pro) or butyrate (But), with signal quantification using ImageJ software. (B) HDAC activity in AGS cells treated with Pro or But. Statistical analysis was performed using one-way ANOVA followed by Tukey’s test. ∗ P < .05, ∗∗ P < .01. (C) ChIP assay showing histone acetylation in the CAPZA1 promoter region of AGS cells treated with Pro or But. Chromatin was immunoprecipitated with anti-histone H3 (acetyl K9) or control IgG, and enrichment of CAPZA1 promoter regions was assessed using real-time PCR. (D) Immunofluorescence analysis of autolysosome formation in But-treated AGS cells infected with H pylori ATCC 700392. Lysosomes and nuclei were stained with LysoTracker and 4ʹ,6-diamidino-2-phenylindole, respectively. Scale bar = 50 μm. (E) Western blotting analysis of CagA accumulation in But-treated AGS cells infected with H pylori ATCC 700392. (F) Western blotting analysis of CD44v expression in Pro- or But-treated AGS cells infected with H pylori G27 or G27 Δ cag PAI strains.

    Journal: Gastro Hep Advances

    Article Title: Helicobacter pylori Exploit Short-Chain Fatty Acids-Induced CAPZA1 Overexpression to Emerge CD44v9-Positive Stemness

    doi: 10.1016/j.gastha.2025.100860

    Figure Lengend Snippet: SCFAs induce CAPZA1 expression in AGS cells and promote CagA accumulation and CD44v expression under H pylori infection. (A) Western blotting analysis of CAPZA1 expression in AGS cells treated with propionate (Pro) or butyrate (But), with signal quantification using ImageJ software. (B) HDAC activity in AGS cells treated with Pro or But. Statistical analysis was performed using one-way ANOVA followed by Tukey’s test. ∗ P < .05, ∗∗ P < .01. (C) ChIP assay showing histone acetylation in the CAPZA1 promoter region of AGS cells treated with Pro or But. Chromatin was immunoprecipitated with anti-histone H3 (acetyl K9) or control IgG, and enrichment of CAPZA1 promoter regions was assessed using real-time PCR. (D) Immunofluorescence analysis of autolysosome formation in But-treated AGS cells infected with H pylori ATCC 700392. Lysosomes and nuclei were stained with LysoTracker and 4ʹ,6-diamidino-2-phenylindole, respectively. Scale bar = 50 μm. (E) Western blotting analysis of CagA accumulation in But-treated AGS cells infected with H pylori ATCC 700392. (F) Western blotting analysis of CD44v expression in Pro- or But-treated AGS cells infected with H pylori G27 or G27 Δ cag PAI strains.

    Article Snippet: Scale bar = 50 μm. (E) Western blotting analysis of CagA accumulation in But-treated AGS cells infected with H pylori ATCC 700392. (F) Western blotting analysis of CD44v expression in Pro- or But-treated AGS cells infected with H pylori G27 or G27 Δ cag PAI strains.

    Techniques: Expressing, Infection, Western Blot, Software, Activity Assay, Immunoprecipitation, Control, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

    SCFAs induce CAPZA1 expression in AGS cells and promote CagA accumulation and CD44v expression under H pylori infection. (A) Western blotting analysis of CAPZA1 expression in AGS cells treated with propionate (Pro) or butyrate (But), with signal quantification using ImageJ software. (B) HDAC activity in AGS cells treated with Pro or But. Statistical analysis was performed using one-way ANOVA followed by Tukey’s test. ∗ P < .05, ∗∗ P < .01. (C) ChIP assay showing histone acetylation in the CAPZA1 promoter region of AGS cells treated with Pro or But. Chromatin was immunoprecipitated with anti-histone H3 (acetyl K9) or control IgG, and enrichment of CAPZA1 promoter regions was assessed using real-time PCR. (D) Immunofluorescence analysis of autolysosome formation in But-treated AGS cells infected with H pylori ATCC 700392. Lysosomes and nuclei were stained with LysoTracker and 4ʹ,6-diamidino-2-phenylindole, respectively. Scale bar = 50 μm. (E) Western blotting analysis of CagA accumulation in But-treated AGS cells infected with H pylori ATCC 700392. (F) Western blotting analysis of CD44v expression in Pro- or But-treated AGS cells infected with H pylori G27 or G27 Δ cag PAI strains.

    Journal: Gastro Hep Advances

    Article Title: Helicobacter pylori Exploit Short-Chain Fatty Acids-Induced CAPZA1 Overexpression to Emerge CD44v9-Positive Stemness

    doi: 10.1016/j.gastha.2025.100860

    Figure Lengend Snippet: SCFAs induce CAPZA1 expression in AGS cells and promote CagA accumulation and CD44v expression under H pylori infection. (A) Western blotting analysis of CAPZA1 expression in AGS cells treated with propionate (Pro) or butyrate (But), with signal quantification using ImageJ software. (B) HDAC activity in AGS cells treated with Pro or But. Statistical analysis was performed using one-way ANOVA followed by Tukey’s test. ∗ P < .05, ∗∗ P < .01. (C) ChIP assay showing histone acetylation in the CAPZA1 promoter region of AGS cells treated with Pro or But. Chromatin was immunoprecipitated with anti-histone H3 (acetyl K9) or control IgG, and enrichment of CAPZA1 promoter regions was assessed using real-time PCR. (D) Immunofluorescence analysis of autolysosome formation in But-treated AGS cells infected with H pylori ATCC 700392. Lysosomes and nuclei were stained with LysoTracker and 4ʹ,6-diamidino-2-phenylindole, respectively. Scale bar = 50 μm. (E) Western blotting analysis of CagA accumulation in But-treated AGS cells infected with H pylori ATCC 700392. (F) Western blotting analysis of CD44v expression in Pro- or But-treated AGS cells infected with H pylori G27 or G27 Δ cag PAI strains.

    Article Snippet: AGS cells were infected with H pylori strains ATCC 700392, G27 ( H pylori G27), or a cag pathogenicity island ( cag PAI)-deleted isogenic mutant ( H pylori G27 Δ cag PAI) at a multiplicity of infection of 50 for 5 h. Following infection, the cells were washed with PBS and subsequently incubated in RPMI 1640 medium containing 400 μg/mL kanamycin for 24 h to kill the bacteria and prevent further injection of CagA.

    Techniques: Expressing, Infection, Western Blot, Software, Activity Assay, Immunoprecipitation, Control, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

    Simplified representation of Helicobacter pylori adhesin-receptor interactions. Le b : Lewis b antigen; sLe a : Sialyl-Lewis an antigen; sLe x : Sialyl-Lewis x antigen; CEACAMs: Carcinoembryonic Antigen-Related Cell Adhesion Molecules; T4SS: Type IV secretion system.

    Journal: World Journal of Gastrointestinal Pathophysiology

    Article Title: Update on the pathogenesis and clinical management of Helicobacter pylori gastric infection and associated diseases

    doi: 10.4291/wjgp.v16.i4.111432

    Figure Lengend Snippet: Simplified representation of Helicobacter pylori adhesin-receptor interactions. Le b : Lewis b antigen; sLe a : Sialyl-Lewis an antigen; sLe x : Sialyl-Lewis x antigen; CEACAMs: Carcinoembryonic Antigen-Related Cell Adhesion Molecules; T4SS: Type IV secretion system.

    Article Snippet: Biological: H. pylori vaccine/biological: Placebo , NCT00613665 , 1 , Completed , Novartis Vaccines.

    Techniques:

    Schematic summary of the underlying mechanisms to Helicobacter pylori -associated gastric diseases. IL-27: Interleukin 27; DupA: Duodenal ulcer promoting protein A; VacA: Vacuolating cytotoxin A; CagA: Cytotoxin-associated antigen A; MALT: Mucosa-associated lymphoid tissue.

    Journal: World Journal of Gastrointestinal Pathophysiology

    Article Title: Update on the pathogenesis and clinical management of Helicobacter pylori gastric infection and associated diseases

    doi: 10.4291/wjgp.v16.i4.111432

    Figure Lengend Snippet: Schematic summary of the underlying mechanisms to Helicobacter pylori -associated gastric diseases. IL-27: Interleukin 27; DupA: Duodenal ulcer promoting protein A; VacA: Vacuolating cytotoxin A; CagA: Cytotoxin-associated antigen A; MALT: Mucosa-associated lymphoid tissue.

    Article Snippet: Biological: H. pylori vaccine/biological: Placebo , NCT00613665 , 1 , Completed , Novartis Vaccines.

    Techniques: